trna碱基修饰检测

• 简介
• 分析种类
• 结果展示

• aksomics（原康成生物）的质谱检测平台能够为客户提供全面而准确的trna修饰谱。利用agilent质谱仪，检测36种修饰碱基在各个样品中的修饰丰度。

  
  

  服务特色：

  ● 优化的前期处理与agilent优质设备 

  ● 全面而准确的碱基修饰分析

  
  

  分析流程：

  

  图释：lc-ms trna修饰检测服务流程图。

  
  

• number	nucleoside	symbol	number	nucleoside	symbol
  01	3′-o-methyladenosine	3′-omea	27	3'-o-methyluridine	3'-omeu
  02	2′-o-methylcytidine	cm	28	5-methyl-2-thiouridine	m5s2u
  03	3-methylcytidine	m3c	29	5-methoxyuridine	mo5u
  04	5-methylcytidine	m5c	30	pseudouridine	ψ
  05	n6-isopentenyladenosine	i6a	31	2'-o-methylinosine	im
  06	5,2'-o-dimethylcytidine	m5cm	32	3-methyluridine	m3u
  07	1-methyladenosine	m1a	33	1-methylpseudouridine	m1ψ
  08	2-thiocytidine	s2c	34	5-hydroxymethylcytidine	hm5c
  09	n2,n2,7-trimethylguanosine	m2,2,7g	35	5,2'-o-dimethyluridine	m5um
  10	n4-acetyl-2'-o-methylcytidine	ac4cm	36	n6-threonylcarbamoyladenosine	t6a
  11	n6-methyladenosine	m6a	37	2-methylthio-n6-threonylcarbamoyladenosine	ms2t6a
  12	3'-o-methylcytidine	3′-omec	38	5-carboxymethyluridine	cm5u
  13	2'-o-methyladenosine	am	39	5-methoxycarbonylmethyl-2-thiouridine	mcm5s2u
  14	n2, n2-dimethylguanosine	m22g	40	5-methoxycarbonylmethyluridine	mcm5u
  15	5'-o-methylthymidine	5′-omet	41	2-methylthio-n6-isopentenyladenosine	ms2i6a
  16	2′-o-methyluridine	um	42	peroxywybutosine	o2yw
  17	inosine	i	43	5-taurinomethyl-2-thiouridine	tm5s2u
  18	2′-o-methylguanosine	gm	44	5-oxyacetic acid uridine	cmo5u
  19	1-methylguanosine	m1g	45	5-carbamoylmethyuridine	ncm5u
  20	7-methylguanosine	m7g	46	queuosine	q
  21	n2-methylguanosin	m2g	47	5-taurinomethyluridine	tm5u
  22	3'-o-methylinosine	3'-omei	48	5-formyl-2′-o-methylcytidine	f5cm
  23	2-thiouridine	s2u	49	dihydrouridine	d
  24	4-thiouridine	s4u	50	5-formylcytidine	f5c
  25	5-methyluridine	m5u	51	wybutosine	yw
  26	n4-acetylcytidine	ac4c	52	5-methoxycarbonylmethyl-2'-o-methyluridine	mcm5um

• 1. 修饰定量分析

  

  图释：trna修饰数量分析图。

  
  

  2. 总离子流程图

  

  图释：总离子流程图