丹麦exiqon公司致力于microrna研究领域,为研究者提供一套完整的microrna研究体系。康成生物提供exiqon芯片技术服务的同时,亦可为您定购mircury lnatm系列产品,包括knockdown探针、northern探针、原位杂交探针和实时定量pcr系列试剂。
mirucry lna knockdown探针标记类型 |
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knockdown probe, ready to label |
knockdown probe, 5’ or 3’ -amino labeled |
knockdown probe, 5’ or 3’-fluorescein labeled |
mircury lna™ microrna原位杂交探针
对mirbase记录和注释的所有物种(包括脊椎动物,无脊椎动物,植物等)的microrna,exiqon均提供相应的lna™ microrna 原位杂交(in situhybridization)探针;探针采用了lna™锁核酸技术并进行了优化,序列特异性强,有效消除二级结构和避免探针自身退火;探针灵敏度高,特异性强,可检测低丰度的microrna;探针标记方式灵活多样,可满足研究者的不同实验需要。
mirucry lna原位杂交检测探针标记类型 |
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in situhybridization detection probe,ready to label |
in situhybridization detection probe,5’ or 3’-dig labeled |
in situhybridization detection probe,5’ or 3’-amino labeled |
in situhybridization detection probe,5’ or 3’-biotin labeled |
in situhybridization detection probe,5’ or 3’-fluorescein labeled |
in situhybridization detection probe,3’ and 5’-dig labeled |
mircury lna™ microrna northern杂交探针
对mirbase记录和注释的所有物种(包括脊椎动物,无脊椎动物,植物等)的microrna,exiqon均提供相应的lna™ microrna northern杂交探针;探针采用了lna™锁核酸技术并进行了优化,序列特异性强,灵敏度高,并有效消除了二级结构和探针自身退火的形成;采用lna™ microrna northern杂交探针,样本总rna最低只需2.5µg。
mirucry lna northern杂交检测探针标记类型 |
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northern blotting detection probe,ready to label |
northern blotting detection probe,5’ or 3’-dig labeled |
northern blotting detection probe,5’ or 3’-amino labeled |
northern blotting detection probe,5’ or 3’-biotin labeled |
northern blotting detection probe,5’ or 3’-fluorescein labeled |
mircurytmlna knockdown探针的主要优点
该图显示的是针对hsa-mir-21的完全配对knockdown探针和错配的knockdown探针的抑制能力。pmir-21报告基因的上调倍数是不同程度的mircurytm lna knockdown错配探针与对照相比得出的结果。实验所用的是hela细胞,转染24小时后用dual-glow™ luciferase检测报告基因的表达水平。
mircurytm 原位杂交检测探针主要优点:
• 高灵敏度,可以检测普通oligo探针检测不到的微量mirnas。
• 覆盖所有已知的mirnas。
• 高特异性,能够区分单碱基或2个碱基差异的mirnas。
• 适应多种标记方法,如dig、生物素、荧光。
• 提供完整的实验步骤。
图 1、mirna-122a是肝细胞特异表达的,本图用mircurytmmirna-122a检测探针对小鼠肝细胞mirna-122a(红色)进行检测。dna(蓝色)用dapi标记。
图2、用mircurytm mirna-124a探针检测人脑颞侧皮质的mirna-124a的表达水平。所用的标本为甲醛固定、石蜡包埋的切片。
product type | product no. | storage temp |
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micury lna microrna inhibitor |
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mircury lna microrna inhibitor, 1 nmol | (4100001-4104908)-000 | -20℃ |
mircury lna microrna inhibitor, 5 nmol | (4100001-4104908)-001 | -20℃ |
mircury lna microrna inhibitor, 15 nmol | (4100001-4104908)-002 | -20℃ |
mircury lna microrna inhibitor, 5 nmol, 5`-fluorescein labeled | (4100001-4104908)-011 | -20℃ |
mircury lna microrna inhibitor, 5 nmol, 3`-fluorescein labeled | (4100001-4104908)-021 | -20℃ |
mircury lna power microrna inhibitor, 1 nmol | (4100001-4104908)-100 | -20℃ |
mircury lna power microrna inhibitor, 5 nmol | (4100001-4104908)-101 | -20℃ |
mircury lna power microrna inhibitor, 15 nmol | (4100001-4104908)-102 | -20℃ |
mircury lna power microrna inhibitor, 5 nmol, 5`-fluorescein labeled | (4100001-4104908)-111 | -20℃ |
mircury lna power microrna inhibitor, 5 nmol, 3`-fluorescein labeled | (4100001-4104908)-121 | -20℃ |
mircury lna microrna inhibitor negative control a, 1 nmol | 199006-000 | -20℃ |
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mircury lna microrna inhibitor negative control a, 5 nmol | 199006-001 | -20℃ |
mircury lna microrna inhibitor negative control a, 15 nmol | 199006-002 | -20℃ |
mircury lna microrna inhibitor negative control a, 5nmol, 5`-fluorescein labeled | 199006-011 | -20℃ |
mircury lna microrna inhibitor negative control a, 5nmol, 3`-fluorescein labeled | 199006-021 | -20℃ |
mircury lna microrna inhibitor negative control b, 1 nmol | 199007-000 | -20℃ |
mircury lna microrna inhibitor negative control b, 5 nmol | 199007-001 | -20℃ |
mircury lna microrna inhibitor negative control b, 15 nmol | 199007-002 | -20℃ |
mircury lna microrna inhibitor negative control b, 5nmol, 5`-fluorescein labeled | 199007-011 | -20℃ |
mircury lna microrna inhibitor negative control b, 5nmol, 3`-fluorescein labeled | 199007-021 | -20℃ |
mircury lna microrna power inhibitor negative control a, 1 nmol | 199006-100 | -20℃ |
mircury lna microrna power inhibitor negative control a, 5 nmol | 199006-101 | -20℃ |
mircury lna microrna power inhibitor negative control a, 15 nmol | 199006-102 | -20℃ |
mircury lna microrna power inhibitor negative control a, 5nmol, 5`-fluorescein labeled | 199006-111 | -20℃ |
mircury lna microrna power inhibitor negative control a, 5nmol, 3`-fluorescein labeled | 199006-121 | -20℃ |
mircury lna microrna power inhibitor negative control b, 1 nmol | 199007-100 | -20℃ |
mircury lna microrna power inhibitor negative control b, 5 nmol | 199007-101 | -20℃ |
mircury lna microrna power inhibitor negative control b, 15 nmol | 199007-102 | -20℃ |
mircury lna microrna power inhibitor negative control b, 5nmol, 5`-fluorescein labeled | 199007-111 | -20℃ |
mircury lna microrna power inhibitor negative control b, 5nmol, 3`-fluorescein labeled | 199007-121 | -20℃ |
product name | product no. | storage temp |
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mircury lna™detection probe, 1 nmol, ready to label | 610000-689999-300 | -20℃ |
mircury lna™detection probe, 1 nmol, 5'-dig labeled | 610000-689999-340 | -20℃ |
mircury lna™detection probe, 1 nmol, 5'-biotin labeled | 610000-689999-370 | -20℃ |
mircury lna™detection probe, 1 nmol, 5'-fluorescein labeled | 610000-689999-310 | -20℃ |
mircury lna™detection probe, 1 nmol, 3'-dig labeled | 610000-689999-350 | -20℃ |
mircury lna™detection probe, 1 nmol, 5'-dig and 3'-dig labeled | 610000-689999-360 | -20℃ |
mircury lna™detection probe, 1 nmol, 5'-fluorescein and 3'-fluorescein labeled | 610000-689999-330 | -20℃ |
product name | product no. | storage temp |
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custom mircury lna™ detection probe, 1 nmol, ready to label | 699999-300 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-dig labeled | 699999-340 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-biotin labeled | 699999-370 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-fluorescein labeled | 699999-310 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-amino labeled | 699999-390 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-tye563 labeled | 699999-330 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-tye665 labeled | 699999-360 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 3'-dig labeled | 699999-350 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 3'-biotin labeled | 699999-380 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 3'-fluorescein labeled | 699999-320 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-dig and 3'-dig labeled | 699998-341 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-biotin and 3'-biotin labeled | 699998-371 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-fluorescein and 3'-fluorescein labeled | 699998-311 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-fluorescein and 4'-fluorescein labeled | 699998-391 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-fluorescein and 5'-fluorescein labeled | 699998-331 | -20℃ |
custom mircury lna™ detection probe, 1 nmol, 5'-fluorescein and 6'-fluorescein labeled | 699998-361 | -20℃ |
product name | product no. | description |
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microrna ish optimization kit 1 (ffpe) | 90001 | optimization kit 1 (mir-1), controls, buffer and protk (400 slides) |
microrna ish optimization kit 2 (ffpe) | 90002 | optimization kit 2 (mir-21), controls, buffer and protk (400 slides) |
microrna ish optimization kit 3 (ffpe) | 90003 | optimization kit 3 (mir-122), controls, buffer and protk (400 slides) |
microrna ish optimization kit 4 (ffpe) | 90004 | optimization kit 4 (mir-124), controls, buffer and protk (400 slides) |
microrna ish optimization kit 5 (ffpe) | 90005 | optimization kit 5 (mir-126), controls, buffer and protk (400 slides) |
microrna ish optimization kit 6 (ffpe) | 90006 | optimization kit 6 (mir-143), controls, buffer and protk (400 slides) |
microrna ish optimization kit 7 (ffpe) | 90007 | optimization kit 7 (mir-145), controls, buffer and protk (400 slides) |
microrna ish optimization kit 8 (ffpe) | 90008 | optimization kit 8 (mir-205), controls, buffer and protk (400 slides) |
microrna ish optimization kit 9 (ffpe) | 90009 | optimization kit 8 (mir-223), controls, buffer and protk (400 slides) |
microrna ish optimization kit 10 (ffpe) | 90010 | controls, buffer and protk (400 slides) |